Protein Science:
Isolation and characterization of proteins (all kinds of liquid chromatography, gel electrophoresis, measurements of enzyme activities, substrate specificity determination ...)
Protein primary and secondary structure analysis (Edman degradation, mass and CD spectrometry) Instructions and Prices
Proteomics (2-dimensional gel electrophoresis, Mudpit, DIGE) Instructions and Price-list
Mass spectrometry Instructions and Price-list
Standard immunological methods (immunocyto- and immunohistochemistry, immunoaffinity chromatography, immunoprecipitation …)
Protein labelling (radio-, fluorescence- and nanoparticle labelling)
Characterization of ligand binding (classical binding studies: competition and equilibrium binding, surface plasmon resonance (SPR), pharmacological profiling)
Molecular and Cell Biology:
Standard methods of molecular biology (recombinant protein production, DNA technology, protein engineering, site-directed mutagenesis, molecular cloning in Escherichia coli ...)
Standard methods of cell biology (cancer, adipose and muscle differentiation cell models, cell proliferation, viability and cytotoxicity assays, epifluorescence- and confocal fluorescence microscopy)
Gene and protein expression analysis and manipulation (gene expression analysis by quantitative real-time PCR, western blot, quantitative proteomics (SILAC), gene downregulation (siRNA/shRNA), overexpression (pDNA transfection), generation of stable cell lines)
Flow cytometry (apoptosis and viability assays, mitochondrial integrity and membrane polarization assays, ROS analysis, lipid droplet analysis, fatty acid uptake, protein expression and transfection analysis)
Lipid-specific cell-based methods (lipid droplet analysis by flow cytometry and microscopy, lipid droplet isolation, free fatty acid, TAG and lipolysis assays, liposomes, quantitative analysis of eicosanoids, resolvins (ELISA, LC/MS lipidomics)
Methods of molecular and cell biology and genetics of yeast
Bioinformatics and Genetics:
Bioinformatics
Standard genetic methods (determination of gene structure and function)
Comparative genomics, phylogenomics and molecular evolution
Transcriptomics (DNA/RNA microarrays …)
Yeast phenomics (synthetic genetic array analysis (SGA and SDL), chemical genomics …)
XQTL method to determine causal genes
⇒ Please contact Prof. Igor Križaj for more information.